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HybSeq

By vojta, 4 August, 2023

Ancient hybridization in Curcuma (Zingiberaceae)—Accelerator or brake in lineage diversifications?

Hybridization is a widespread phenomenon in the evolution of plants and exploring its role is crucial to understanding diversification processes of many taxonomic groups. Recently, more attention is focused on the role of ancient hybridization that has repeatedly been shown as triggers of evolutionary radiation, although in some cases, it can prevent further diversification. The causes, frequency, and consequences of ancient hybridization remain to be explored.

Články
Bioinformatics
Institute of Botany, Czech Academy of Sciences
Phylogeny
HybSeq
Faculty of Science, Charles University
Science, research, biology
By vojta, 21 June, 2023

Phylogenetic challenges in a recently diversified and polyploid-rich Alyssum (Brassicaceae) lineage: low divergence, reticulation, and parallel polyploid speciation

Elucidating the evolution of recently diverged and polyploid-rich plant lineages may be challenging even with high-throughput sequencing, both for biological reasons and bioinformatic difficulties. Here, we apply target enrichment with genome skimming (Hyb-Seq) to unravel the evolutionary history of the Alyssum montanum-A. repens species complex. Reconstruction of phylogenetic relationships in diploids supported recent and rapid diversification accompanied by reticulation events.

Články
Arabidopsis and Brassicaceae
Bioinformatics
Institute of Botany, Czech Academy of Sciences
Phylogeny
Phylogeography
HybSeq
Faculty of Science, Charles University
Taxonomy
Science, research, biology
By vojta, 29 May, 2023

Target enrichment in taxonomy, HybSeq course 2023

Practical course of phylogenomic methods focused on Hyb-Seq NGS method. The Hyb-Seq method combines target enrichment and genome skimming of the genome. The course covers the theory, discussion of the newest papers from the field, probe design, laboratory and computer part. Several methodological approaches to data analysis, from raw data to species tree analysis, are demonstrated within the course. The lab part includes NGS library preparation (using sonicator) and enrichment.

Články
Bioinformatics
Phylogeny
HybSeq
Linux (and another software)
Faculty of Science, Charles University
Science, research, biology
Teaching
By vojta, 12 October, 2022

The relationship between transposable elements and ecological niches in the Greater Cape Floristic Region: A study on the genus Pteronia (Asteraceae)

Non-coding repetitive DNA (repeatome) is an active part of the nuclear genome, involved in its structure, evolution and function. It is dominated by transposable elements (TEs) and satellite DNA and is prone to the most rapid changes over time. The TEs activity presumably causes the global genome reorganization and may play an adaptive or regulatory role in response to environmental challenges.

Články
Bioinformatics
Institute of Botany, Czech Academy of Sciences
Phylogeny
HybSeq
Science, research, biology
By vojta, 3 June, 2022

Target enrichment for plant systematics - HybSeq course 2022

Practical course of phylogenomic methods focused on Hyb-Seq NGS method. The Hyb-Seq method combines target enrichment and genome skimming of the genome. The course covers the theory, discussion of the newest papers from the field, probe design, laboratory and computer part. Several methodological approaches to data analysis, from raw data to species tree analysis, are demonstrated within the course. The lab part includes NGS library preparation (using sonicator) and enrichment.

Články
Bioinformatics
Phylogeny
HybSeq
Linux (and another software)
Faculty of Science, Charles University
Science, research, biology
Teaching
By vojta, 26 July, 2021

Relative performance of customized and universal probe sets in target enrichment: A case study in subtribe Malinae

Custom probe design for target enrichment in phylogenetics is tedious and often hinders broader phylogenetic synthesis. The universal angiosperm probe set Angiosperms353 may be the solution. Here, we test the relative performance of Angiosperms353 on the Rosaceae subtribe Malinae in comparison with custom probes that we specifically designed for this clade. We then address the impact of bioinformatically altering the performance of Angiosperms353 by replacing the original probe sequences with orthologs extracted from the Malus domestica genome.

Články
Bioinformatics
Institute of Botany, Czech Academy of Sciences
Phylogeny
HybSeq
Linux (and another software)
Faculty of Science, Charles University
Taxonomy
Science, research, biology
By vojta, 19 December, 2019

HybSeq course 2020

Intensive 4-days (5th day is not compulsory, but is open for any discussion, if there would be interest) course to learn all theory about HybSeq and practically learn how to analyze HybSeq data, how to solve all problems, and how to evaluate differences among gene trees. Important part is enough time to discuss everything, including practical problems and projects of individual participants.

Články
Bioinformatics
HybSeq
Linux (and another software)
Faculty of Science, Charles University
Science, research, biology
Teaching
By vojta, 2 August, 2018

Hunting the treasure of Cape Oxalis diversity

Poster presented at Botany 2018 conference of the Botanical Society of America titled Hunting the treasure of Cape Oxalis diversity (Zeisek, Oberlander, Dreyer, Schmickl, Schneeweiss):

Články
Bioinformatics
Institute of Botany, Czech Academy of Sciences
Phylogeny
HybSeq
Oxalis
Faculty of Science, Charles University
Taxonomy
Science, research, biology
By vojta, 26 November, 2015

Phylogenetic marker development for target enrichment from transcriptome and genome skim data: the pipeline and its application in southern African Oxalis (Oxalidaceae)

Phylogenetics benefits from using a large number of putatively independent nuclear loci and their combination with other sources of information, such as the plastid and mitochondrial genomes. To facilitate the selection of orthologous low-copy nuclear (LCN) loci for phylogenetics in non-model organisms, we created an automated and interactive script to select hundreds of LCN loci by a comparison between transcriptome and genome skim data. We used our script to obtain LCN genes for southern African Oxalis (Oxalidaceae), a speciose plant lineage in the Greater Cape Floristic Region.

Články
Bioinformatics
Phylogeny
HybSeq
Linux (and another software)
Oxalis
Faculty of Science, Charles University
Taxonomy
Science, research, biology
HybSeq
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This work by Vojtěch Zeisek is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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