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By vojta , 21 June 2023

Phylogenetic challenges in a recently diversified and polyploid-rich Alyssum (Brassicaceae) lineage: low divergence, reticulation, and parallel polyploid speciation

Elucidating the evolution of recently diverged and polyploid-rich plant lineages may be challenging even with high-throughput sequencing, both for biological reasons and bioinformatic difficulties. Here, we apply target enrichment with genome skimming (Hyb-Seq) to unravel the evolutionary history of the Alyssum montanum-A. repens species complex. Reconstruction of phylogenetic relationships in diploids supported recent and rapid diversification accompanied by reticulation events.

Články
Arabidopsis and Brassicaceae
Bioinformatics
Institute of Botany, Czech Academy of Sciences
Phylogeny
Phylogeography
HybSeq
Faculty of Science, Charles University
Taxonomy
Science, research, biology
By vojta , 29 May 2023

Target enrichment in taxonomy, HybSeq course 2023

Practical course of phylogenomic methods focused on Hyb-Seq NGS method. The Hyb-Seq method combines target enrichment and genome skimming of the genome. The course covers the theory, discussion of the newest papers from the field, probe design, laboratory and computer part. Several methodological approaches to data analysis, from raw data to species tree analysis, are demonstrated within the course. The lab part includes NGS library preparation (using sonicator) and enrichment.

Články
Bioinformatics
Phylogeny
HybSeq
Linux (and another software)
Faculty of Science, Charles University
Science, research, biology
Teaching
By vojta , 31 December 2022

PF 2023

Pour féliciter 2023!

PF 2023

Články
Others
By vojta , 12 October 2022

Course of work with molecular data in R 2023

R is nowadays probably the most powerful tool for calculations of all kinds. There are plenty of modules available for work with molecular data. Their representative selection will be introduced during the course.

The course contains theory of used methods, tutorials with test data, tasks for individual work of participants, and more. The aim is to teach students how to analyze molecular data in R programming language, introduce available packages for their analysis and practical trying out to work with own or provided data.

Články
Phylogeny
Phylogeography
Linux (and another software)
Population genetics
Faculty of Science, Charles University
R
Taxonomy
Teaching
By vojta , 12 October 2022

Course of work in Linux command line not only for MetaCentrum 2023

Don’t be afraid of command line! It is friendly and powerful tool allowing to process large data and automate tasks. Practically identical is command line also in Apple macOS, BSD and another UNIX-based systems, not only in Linux. The course is designed for total beginners as well as intermediate advanced students. The only requirement is an interest (or need) to work in command line, typically on Linux computing server.

Články
Linux (and another software)
openSUSE
Faculty of Science, Charles University
Teaching
By vojta , 12 October 2022

The relationship between transposable elements and ecological niches in the Greater Cape Floristic Region: A study on the genus Pteronia (Asteraceae)

Non-coding repetitive DNA (repeatome) is an active part of the nuclear genome, involved in its structure, evolution and function. It is dominated by transposable elements (TEs) and satellite DNA and is prone to the most rapid changes over time. The TEs activity presumably causes the global genome reorganization and may play an adaptive or regulatory role in response to environmental challenges.

Články
Bioinformatics
Institute of Botany, Czech Academy of Sciences
Phylogeny
HybSeq
Science, research, biology
By vojta , 12 October 2022

The importance of considering the evolutionary history of polyploids when assessing climatic niche evolution

Although whole-genome duplication (WGD) is an important speciation force, we still lack a consensus on the role of niche differentiation in polyploid evolution. In addition, the role of genome doubling per se vs. later divergence on polyploid niche evolution remains obscure. One reason for this might be that the intraspecific genetic structure of polyploid complexes and interploidy gene flow is often neglected in ecological studies. Here, we aim to investigate to which extent these evolutionary processes impact our inference on niche differentiation of autopolyploids.

Články
Arabidopsis and Brassicaceae
Bioinformatics
Institute of Botany, Czech Academy of Sciences
Phylogeography
Population genetics
Faculty of Science, Charles University
Science, research, biology
By vojta , 3 June 2022

Target enrichment for plant systematics - HybSeq course 2022

Practical course of phylogenomic methods focused on Hyb-Seq NGS method. The Hyb-Seq method combines target enrichment and genome skimming of the genome. The course covers the theory, discussion of the newest papers from the field, probe design, laboratory and computer part. Several methodological approaches to data analysis, from raw data to species tree analysis, are demonstrated within the course. The lab part includes NGS library preparation (using sonicator) and enrichment.

Články
Bioinformatics
Phylogeny
HybSeq
Linux (and another software)
Faculty of Science, Charles University
Science, research, biology
Teaching
By vojta , 11 January 2022

Virtual machine for my courses

For my courses ofwork in Linux command line not only for MetaCentrum and with molecular data in R I provide VirtualBox image, which allows to run complete desktop Linux (in this case openSUSE Leap) with all preinstalled applications needed for both courses. It's easy way how to get fully working Linux to play with. It requires at least bit powerful notebook, e.g. at least quad-core with at least 8 GB RAM, but more is better.

Články
Bioinformatics
Linux (and another software)
openSUSE
Faculty of Science, Charles University
Teaching
By vojta , 11 November 2021

Course of work with molecular data in R 2022

R is nowadays probably the most powerful tool for calculations of all kinds. There are plenty of modules available for work with molecular data. Their representative selection will be introduced during the course.

The course contains theory of used methods, tutorials with test data, tasks for individual work of participants, and more. The aim is to teach students how to analyze molecular data in R programming language, introduce available packages for their analysis and practical trying out to work with own or provided data.

Články
Phylogeny
Phylogeography
Linux (and another software)
Population genetics
Faculty of Science, Charles University
R
Taxonomy
Teaching

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