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Taxonomy

By vojta, 21 June, 2023

Phylogenetic challenges in a recently diversified and polyploid-rich Alyssum (Brassicaceae) lineage: low divergence, reticulation, and parallel polyploid speciation

Elucidating the evolution of recently diverged and polyploid-rich plant lineages may be challenging even with high-throughput sequencing, both for biological reasons and bioinformatic difficulties. Here, we apply target enrichment with genome skimming (Hyb-Seq) to unravel the evolutionary history of the Alyssum montanum-A. repens species complex. Reconstruction of phylogenetic relationships in diploids supported recent and rapid diversification accompanied by reticulation events.

Články
Arabidopsis and Brassicaceae
Bioinformatics
Institute of Botany, Czech Academy of Sciences
Phylogeny
Phylogeography
HybSeq
Faculty of Science, Charles University
Taxonomy
Science, research, biology
By vojta, 12 October, 2022

Course of work with molecular data in R 2023

R is nowadays probably the most powerful tool for calculations of all kinds. There are plenty of modules available for work with molecular data. Their representative selection will be introduced during the course.

The course contains theory of used methods, tutorials with test data, tasks for individual work of participants, and more. The aim is to teach students how to analyze molecular data in R programming language, introduce available packages for their analysis and practical trying out to work with own or provided data.

Články
Phylogeny
Phylogeography
Linux (and another software)
Population genetics
Faculty of Science, Charles University
R
Taxonomy
Teaching
By vojta, 11 November, 2021

Course of work with molecular data in R 2022

R is nowadays probably the most powerful tool for calculations of all kinds. There are plenty of modules available for work with molecular data. Their representative selection will be introduced during the course.

The course contains theory of used methods, tutorials with test data, tasks for individual work of participants, and more. The aim is to teach students how to analyze molecular data in R programming language, introduce available packages for their analysis and practical trying out to work with own or provided data.

Články
Phylogeny
Phylogeography
Linux (and another software)
Population genetics
Faculty of Science, Charles University
R
Taxonomy
Teaching
By vojta, 26 July, 2021

Relative performance of customized and universal probe sets in target enrichment: A case study in subtribe Malinae

Custom probe design for target enrichment in phylogenetics is tedious and often hinders broader phylogenetic synthesis. The universal angiosperm probe set Angiosperms353 may be the solution. Here, we test the relative performance of Angiosperms353 on the Rosaceae subtribe Malinae in comparison with custom probes that we specifically designed for this clade. We then address the impact of bioinformatically altering the performance of Angiosperms353 by replacing the original probe sequences with orthologs extracted from the Malus domestica genome.

Články
Bioinformatics
Institute of Botany, Czech Academy of Sciences
Phylogeny
HybSeq
Linux (and another software)
Faculty of Science, Charles University
Taxonomy
Science, research, biology
By vojta, 25 February, 2021

STRUCTURE multi PBS Pro scripts

Set of scripts to run STRUCTURE in parallel on computing grids like MetaCentrum. Scripts are designed for grids and clusters using PBS Pro, but can be easily adopted for another queue system.

Články
Bioinformatics
Institute of Botany, Czech Academy of Sciences
Phylogeny
Phylogeography
Linux (and another software)
Population genetics
Faculty of Science, Charles University
Taxonomy
Science, research, biology
By vojta, 6 October, 2020

Course of work with molecular data in R 2021

R is nowadays probably the most powerful tool for calculations of all kinds. There are plenty of modules available for work with molecular data. Those will be introduced during the course.

The course contains theory of used methods, tutorials with test data, tasks for individual work of participants, and more.

Previous knowledge of R is useful, but not necessary. At least basic knowledge of molecular biology is required, previous knowledge about any methods how to analyse DNA data is recommended.

Články
Phylogeny
Phylogeography
Linux (and another software)
Population genetics
Faculty of Science, Charles University
R
Taxonomy
Teaching
By vojta, 6 March, 2020

Course of work with molecular data in R 2020 in České Budějovice

R is nowadays probably the most powerful tool for calculations of all kinds. There are plenty of modules available for work with molecular data. Those will be introduced during the course. The course will be taught from October 19th to Friday 23rd (see below). The course will be exclusively on-line, there will be no personal meeting.

Články
Phylogeny
Phylogeography
Linux (and another software)
Population genetics
Faculty of Science, Charles University
R
Taxonomy
Teaching
By vojta, 13 January, 2020

Low genetic differentiation despite high fragmentation in the endemic serpentinophyte Minuartia smejkalii (M. verna agg., Caryophyllaceae) revealed by RADSeq SNP markers

Minuartia smejkalii is an obligate serpentinophyte plant endemic to the Czech Republic. Since the 1960s, the species’ habitat has undergone strong human-mediated fragmentation, resulting in extinction of some populations and dramatic size reduction of the remaining populations. Thus, contrary to the typically stable serpentine habitats, M. smejkalii habitats underwent a recent and severe decline, which can exacerbate the effects of fragmentation on population genetic structure. We examined the genetic structure of all known M.

Články
Institute of Botany, Czech Academy of Sciences
Phylogeography
Population genetics
Taxonomy
Science, research, biology
By vojta, 7 October, 2019

Course of work with molecular data in R 2020

R is nowadays probably the most powerful tool for calculations of all kinds. There are plenty of modules available for work with molecular data. Those will be introduced during the course.

The course contains theory of used methods, tutorials with test data, tasks for individual work of participants, and more.

Previous knowledge of R is useful, but not necessary. At least basic knowledge of molecular biology is required, previous knowledge about any methods how to analyse DNA data is recommended.

Články
Phylogeny
Phylogeography
Linux (and another software)
Population genetics
Faculty of Science, Charles University
R
Taxonomy
Teaching
By vojta, 29 May, 2019

Analysis of wild Taraxacum bicorne Dahlst. (Compositae–Crepidinae) as a potential alternative natural rubber crop

Taraxacum koksaghyz Rodin, a dandelion from the steppes of south-eastern Kazakhstan, has been known for long time as potential rubber producer, as a temperate region alternative to the tropical rubber tree Hevea brasiliensis Müll. Arg.. In this work, we evaluate Taraxacum bicorne Dahlst. (wild populations), a closely related congener of T. koksaghyz.

Články
Institute of Botany, Czech Academy of Sciences
Population genetics
Taraxacum
Taxonomy
Science, research, biology

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