Relative performance of customized and universal probe sets in target enrichment: A case study in subtribe Malinae

Submitted by vojta on Mon, 07/26/2021 - 12:46

Custom probe design for target enrichment in phylogenetics is tedious and often hinders broader phylogenetic synthesis. The universal angiosperm probe set Angiosperms353 may be the solution. Here, we test the relative performance of Angiosperms353 on the Rosaceae subtribe Malinae in comparison with custom probes that we specifically designed for this clade. We then address the impact of bioinformatically altering the performance of Angiosperms353 by replacing the original probe sequences with orthologs extracted from the Malus domestica genome.

STRUCTURE multi PBS Pro scripts

Set of scripts to run STRUCTURE in parallel on computing grids like MetaCentrum. Scripts are designed for grids and clusters using PBS Pro, but can be easily adopted for another queue system.

vojta Thu, 02/25/2021 - 17:24

PF 2021

Submitted by vojta on Fri, 01/01/2021 - 21:34

Pour féliciter 2021!

PF 2021


Parallel Alpine Differentiation in Arabidopsis arenosa

Submitted by vojta on Tue, 12/29/2020 - 11:12

Parallel evolution provides powerful natural experiments for studying repeatability of evolution and genomic basis of adaptation. Well-documented examples from plants are, however, still rare, as are inquiries of mechanisms driving convergence in some traits while divergence in others. Arabidopsis arenosa, a predominantly foothill species with scattered morphologically distinct alpine occurrences is a promising candidate. Yet, the hypothesis of parallelism remained untested.

Course of work with molecular data in R 2021

Submitted by vojta on Tue, 10/06/2020 - 17:08

R is nowadays probably the most powerful tool for calculations of all kinds. There are plenty of modules available for work with molecular data. Those will be introduced during the course.

The course contains theory of used methods, tutorials with test data, tasks for individual work of participants, and more.

Previous knowledge of R is useful, but not necessary. At least basic knowledge of molecular biology is required, previous knowledge about any methods how to analyse DNA data is recommended.

Course of work in Linux command line not only for MetaCentrum 2021

Submitted by vojta on Tue, 10/06/2020 - 16:49

Don’t be afraid of command line! It is friendly and powerful tool allowing to process large data and automate tasks. Practically identical is command line also in Apple macOS, BSD and another UNIX-based systems, not only in Linux. The course is designed for total beginners as well as intermediate advanced students. The only requirement is an interest (or need) to work in command line, typically on Linux computing server.

Course will be taught in Linux, but most of the point are applicable also for another UNIX systems like macOS.

Course of work with molecular data in R 2020 in České Budějovice

R is nowadays probably the most powerful tool for calculations of all kinds. There are plenty of modules available for work with molecular data. Those will be introduced during the course. The course will be taught from October 19th to Friday 23rd (see below). The course will be exclusively on-line, there will be no personal meeting.

vojta Fri, 03/06/2020 - 22:39
Low genetic differentiation despite high fragmentation in the endemic serpentinophyte Minuartia smejkalii (M. verna agg., Caryophyllaceae) revealed by RADSeq SNP markers

Minuartia smejkalii is an obligate serpentinophyte plant endemic to the Czech Republic. Since the 1960s, the species’ habitat has undergone strong human-mediated fragmentation, resulting in extinction of some populations and dramatic size reduction of the remaining populations. Thus, contrary to the typically stable serpentine habitats, M. smejkalii habitats underwent a recent and severe decline, which can exacerbate the effects of fragmentation on population genetic structure. We examined the genetic structure of all known M.

vojta Mon, 01/13/2020 - 10:46

PF 2020

Submitted by vojta on Sun, 12/22/2019 - 14:54

Pour féliciter 2020!

PF 2020


HybSeq course 2020

Submitted by vojta on Thu, 12/19/2019 - 21:25

Intensive 4-days (5th day is not compulsory, but is open for any discussion, if there would be interest) course to learn all theory about HybSeq and practically learn how to analyze HybSeq data, how to solve all problems, and how to evaluate differences among gene trees. Important part is enough time to discuss everything, including practical problems and projects of individual participants.